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*AccuQuik™ RT-PCR COVID-19 Test Kit is CE, ISO, USFDA certified.

*AccuQuik™ RT-PCR COVID-19 Test Kit is manufactured in our facilities located in China/India/USA.

(choose the specifications you require below)
Test:

RT-PCR SARS-CoV-2 Test

Form:

Tubes for Laboratory PCR Machinery

Packaging:

25 tests/kit, 1 kit/box

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Product Information

AdvaCare is a CE, ISO and USFDA manufacturer of RT-PCR COVID-19 (Coronavirus) Nucleic Acid Test Kit.

AccuQuik™ RT-PCR SARS-CoV-2 Nucleic Acid Test Kit is a real-time RT-PCR test intended for the qualitative detection of nucleic acid from the SARS-CoV-2, novel coronavirus which was identified in 2019 at Wuhan City, and from there spread around the globe. Novel coronavirus (SARS-CoV-2) can be identified via multiplex PCR-fluorescent probe technology in upper and lower respiratory tract specimens (such as oropharyngeal swabs, nasopharyngeal swabs and sputum).

Thanks to its designed dual-target gene detection primer probe, our AccuQuik™ RT-PCR COVID-19 kit works with a high-efficiency RT-PCR reaction system for detection. After the end of the reaction, the results will be determined through analysis of the cycle threshold (Ct) of each channel. Moreover, the kit is designed with human housekeeping gene RNaseP for monitoring sampling, extraction, specimen addition, amplification and other related processes as an internal standard to effectively prevent false positive and false negative results, so as to ensure the specificity and accuracy of the test results.

Polymerase chain reaction (PCR) is a method used in molecular biology: by multiplying DNA samples PCR method amplifies in a more manageable size for study and analysis.

Reverse transcription polymerase chain reaction (RT-PCR) is a laboratory technique combining reverse transcription of RNA into DNA and amplification of specific DNA targets using PCR method. This allows to analyze gene expression and quantification of viral RNA in specimens.

Additional Information

Important Notice for Distributors

We, at AdvaCare, have been working around the clock to address this global pandemic, with a special focus on the early detection of COVID-19, to expedite development of our revolutionary testing solution. We stand on the front lines in the fight against novel coronavirus (COVID-19)!

As AdvaCare production lines are running at full capacity to meet demand, delivery times are according to when orders are placed.

Contact us today to Receive Prices, Specifications and Shipment Schedule.

Specimen Collection Method

Oropharyngeal Swabs:

  • Simultaneously wipe the bilateral pharyngeal tonsils and the posterior pharyngeal wall with two plastic rod swabs with a polypropylene fiber head;
  • Immerse the swab head in a tube containing 3ml of samplig solution;
  • Discard tail of swab and tighten the tube cap;
  • Put the tube into biohazard bag for transportation.

Nasopharyngeal Swabs:

  • Insert a plastic rod swab witha polypropyulene fiber head in the nasal passage to reach the nasopalatine duct gently;
  • Allow swab to stay for a while and slowly rotate it to exit;
  • Take another plastic rod swabt with a polypropylene fiber head and collect the specimen in the other nostril in the same way;
  • Immerse the two swabs mentioned above in the same tube containing 3ml of sampling solution;
  • Discard tail of swab and tighten the tube cap;
  • Put the tube into biohazard bag for transportation.

AdvaCare suggests the usage of our AccuQuik™ PCR Virus Transportation System.

Sputum:

  • Collect the sputum coughed up into a 50ml screw-on plastic tube containing 3ml of sampling solution;
  • Put the tube into biohazard bag for transportation.

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Precautions

  1. Please refer to the entire Package Instruction before using the kit;
  2. This product does not contain nucleic acid extraction reagent, which needs to be prepared by the user;
  3. Please stricktly follow the management practices of genetics amplification testing laboratories as issued by the industrial administrative department in charge.
  4. This kit is an in vitro detection reagent, and the operating personnel must be professionally trained;
  5. The entire detection process shall be strictly conducted in three areas, i.e., Reagent Preparation Area, Specimen Preparation Area and Amplification Area. The instruments, equipment, consumables and work clothes used in each area shall be independent and dedicated. The workbench shall be cleaned immediately after the experiment and be disinfected;
  6. Please use disposable dedicated centrifuge tubes, calibrated pipettes, tips with filter element, as well as disposable gloves that do not contain fluorescent materials. Gloves must be replaced frequently;
  7. The components in the kit shall be used within the validity period. Do not mix reagents of different batches;
  8. All reagents must be thawed completely, mixed well and centrifuged at 6,000rpm for a few seconds before use;
  9. Instruments and equipment, such as operating tables, pipettes, centrifuges and PCR analyzer, shall be disinfected frequently with 10% sodium hypochlorite or 75% alcohol, UV or ozone;
  10. Try to avoid air bubbles in the reaction tube. The tube cap must be tightened;
  11. After the amplification is completed, take out the reaction tube, seal it in a special plastic bag, and discard it at the designated place;
  12. The test specimens involved with this kit are considered to be infectious substances, and any operation and processing must meet the relevant requirements of General Biosafety Standard for Microbiological and Biomedical Laboratories and Medical Waste Management Regulations released by the Ministry of Health of the P.R.C.;
  13. Test kit for professional use only.

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