Myoglobin, creatine kinase MB and cardiac troponin I proteins get released after a cardiac injury into the bloodstream. Myoglobin is a heme protein, and it presents the 2% of the total muscle protein that transports oxygen within muscle cells. Two-four hours after injury the myoglobin baseline increases. It peaks after nine to twelve hours.
CK-MB is involved in cardiac muscle tissue metabolism that can be detected 3 to 8 hours after myocardial infarction, with a peak after around 9 to 30 hours. Troponin I is found in cardiac muscle. After suffering from an injury 4 to 6 hours later Troponin I is released into the blood and remains elevated for 6 to 10 days.
Cardiac proteins work as biomarkers that are used to diagnose for example heart attack. Myoglobin found in blood could also be from skeletal muscle but a troponin test will confirm if it is from the heart or not.
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- They are available, affordable, and usable as a point of care tests.
Bring tests and the specimen to room temperature (between 15 to 30°C) before use. Then remove the test from its sealed pouch. It is advisable to use it as soon as possible, and for the best results, the test should be performed within one hour. When performing the test, place the kit on a clean and level surface.
If using serum or plasma specimens, hold vertically and transfer 2 drops of serum or plasma (50μl) to the specimen well of the test device, then start the timer. Hold the strip by the end, where the product name is printed. To avoid contamination, do not touch the strip membrane.
Another option is to transfer 3 drops of whole blood specimen (75μl) to the specimen well of the device with the provided disposable pipette and then add 1 drop of buffer before starting the timer.
The last option is also to allow 3 hanging drops of fingerstick whole blood specimen to fall into the center of the specimen well on the device, after which you should add 1 drop of buffer and start the timer.
Avoid trapping air bubbles in the specimen well, and do not add any solution to the result area. As the test begins to work, the color will migrate across the membrane. Before reading the results, wait for the colored band to appear. The results should be read at 10 minutes. Do not interpret the result after 20 minutes.
- For professional in vitro diagnostic use only;
- Do not use after expiration date;
- The test must remain in the sealed pouch until use;
- Do not use if pouch is damaged;
- Handle all specimens as if they contain infectious agents.