Urinalysis Test Strips are used to detect a broad range of diseases. The test is designed to identify proteins, sugars, organic compounds, bacteria, chemicals that are usually found in red blood cells, and white blood cell count. It also tests the acidity and mass reaction of pathogens. Urinalysis test strips are used for screening in high-risk areas and for monitoring treatment. These test strips can be used to rule out possible infections and diseases.
AccuQuik Urinalysis Test Strips can detect leukocyte, nitrite, urobilinogen, protein, pH, blood, specific gravity, ketone, bilirubin, glucose, and ascorbic acid in urine. It can indicate the presence of infection of the kidneys, ureters, bladder or urethra, kidney or urinary tract disease, liver cell disease, biliary obstruction, diabetes, and other diseases or infections. If you receive a test result that indicates an abnormal level of any of the eleven factors, consult a doctor to determine what, if any, action is required.
Leukocytes: This test reveals the presence of granulocyte esterases. The esterases cleave a derivatized thiazole amino acid ester to liberate derivatized hydroxyl thiazole. This thiazole then reacts with a diazonium salt to produce a purple product.
Nitrite: This test is based on the reaction of p-arsanilic acid and nitrite (which is derived from dietary nitrate in the presence of bacteria) in urine to form a diazonium compound. The diazonium compound in turn couples with N-(1-naphthyl)ethylenediamine in an acidic medium. The resulting color is pink. Any degree of pink color is considered positive.
Urobilinogen: The test is based on the diazotization reaction of 4-Methoxy-benzene diazonium salt and urinary urobilinogen in a strong acid medium. The color changes range from light tan to brown-red.
Protein: This test is based on the color change of the indicator, tetrabromophenol blue, in the presence of protein. A positive reaction is indicated by a color change from yellow through green and then to greenish-blue.
pH: This test is based on double indicators (methyl red and bromothymol blue) which give a broad range of colors covering the entire urinary pH range. Colors range from orange through greenish-yellow and green to blue.
Blood: This test is based on the pseudoperoxidase activity of hemoglobin which catalyzes the reation of 3,3’5.5’-tetramethylbenzidine and buffered organic peroxide, 2,5-dimethylhexane-2,5-dihydroperoxide. The resulting color ranges from greenish-yellow through bluish-green to dark blue.
Specific Gravity: This test is based on the pka change. In the presence of cations, protons are released by a complexing agent and produce a color change in the indicator bromothymol blue via blue-green to yellow.
Ketones: This test is based on the reaction of acetoacetic acid in the urine with nitroprusside. The resulting color ranges from tan when no reaction takes place to purple for positive reaction.
Bilirubin: This test is based on the coupling of bilirubin with 2,4-dichloro-benzene diazonium salt in a strong acid medium. The color changes from light tan to pinkish-purple.
Glucose: This test is based on a sequential enzyme reaction. First, glucose oxidase catalyzes the formation of gluconic acid and hydrogen peroxide from the oxidation of glucose. A second enzyme, peroxidase, catalyzes the reaction of hydrogen peroxide with protassium iodide chromogen to oxidize the chromogen to colors ranging from blue through greenish-brown to dark brown.
Ascorbic Acid: This test reveals the concentration of ascorbic acid in urine which varies with the intake. It is approximately half of the intake.
Allow the test strip to reach room temperature (15-30°C) prior to testing. Do not open the bottle until ready to perform the assay.
Take out the test strip from the bottle and replace the cap immediately. Immerse the reagent pads of the test strip completely into the sample of urine and remove immediately.
Lean the test strip against the edge of the container side to wipe away extra urine.
Hold the test strip, compare it with the colorimetric table on the bottle, and record the test results in its determination time, which vary from factor to factor.