Rotavirus causes diarrhea in infants and young children. Rotavirus enters through the mouth and infects intestinal lining. It can spread through contaminated surfaces, hands and objects and is, thus, highly contagious. A person can, therefore, get sick after touching a contaminated object or by consuming food or drinks with contaminated hands.
After 1 to 3 days of exposure, the usual symptoms may occur but are not limited to vomiting, fever, diarrhea, abdominal pain, fever and loss of appetite. Rotavirus resolves after 3 to 9 days of symptoms. Severe dehydration for young children can be fatal.
There is no specific treatment for rotavirus infection. Vaccines can prevent infants and young children from being infected. Handwashing is important for controlling the spread of the infection.
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Firstly, bring all materials and specimen to room temperature (8 to 30°C). Continue by removing the test cassette from the sealed foil pouch. Then, hold the sample bottle upright with the tipping point toward the direction away from the test performer, and snap off the tip.
Secondly, hold the bottle in a vertical position over the sample well of the test card, and deliver 3 drops (120-150μL) of the diluted stool sample to the sample well. Wait and read the results after 5 to 10 minutes. A strong positive sample may influence the results to show earlier. Keep in mind, that the results after 10 minutes may not be accurate.
- Never freeze the tests. If the test is stored in the refrigerator, the test should be brought down to room temperature before being used;
- Once the test procedure starts, every step should be taken uninterruptedly;
- Do not eat, drink or smoke in the area where the specimens or kits are handled;
- Humidity and temperature can adversely affect results;
- The number of drops to be added should be observed carefully. Only 3 to 4 drops should be added in the test.