NT-proBNP is a prohormone with a 76 amino acid N-terminal inactive protein that is activated to release brain natriuretic peptide (BNP). Brain Natriuretic Peptide (BNP) is released by cardiomyocytes in heart ventricles when the ventricular blood volume is increased. BNP decreases systemic vascular resistance and central venous pressure and natriuresis is increased. BNP and NT-proBNP levels in the blood are used to determine acute congestive heart failure and also for the prognosis of heart failure. Higher levels of BNP and NT-proBNP are typical for patients with symptomatic and asymptomatic left ventricular dysfunction and is associated with coronary artery disease and myocardial ischemia.
Shortness of breath and fatigue occurs in patients with reduced cardiac output. NT-proBNP improves diagnostic accuracy in patients with shortness of breath and potential heart failure.
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- Results available in only 10 minutes;
- Easy to use;
- No need for an analyser;
- Qualitative results can be read visually;
- Cost effective method for identifying heart failure in patients.
Bring the test, specimen, buffer, and controls to reach room temperature 15 to 30°C before testing. Open a pouch and remove the cassette from the pouch. Use it within one hour on a clean and flat surface.
If using serum or plasma specimen, transfer 3 drops of serum or plasma (75μl) to the sample well of the test cassette. If using Venipuncture Whole Blood specimen, transfer 3 drops of whole blood (75 μl) to the specimen well and add 1 drop of buffer (40 μl).
If using Fingerstick Whole Blood specimen, use a capillary tube, fill it and transfer 75 μl of fingerstick whole blood specimen to the specimen well and add 1 drop of buffer (40 μl). To use hanging drops allow 3 hanging drops of fingerstick whole blood specimen (75 μl) into the specimen well.
Read the results at 10 minutes but not after 20 minutes.
The test result is positive if there is a colored line in the control line region and there is also another line that says that the result is positive. More intense color depends on the concentration of NT-proBNP in the specimen. Negative results are detected when there is only 1 line. Invalid results appear when no lines are present.
- Do not use if pouch is damaged;
- Do not eat, drink or smoke in the area where the specimens or kits are handled;
- Wear protective clothing such as laboratory coats, disposable gloves or eye protection when specimens are being tested;
- Humidity and temperature can adversely affect results;
- The used test should be discarded according to local regulations.