Cardiac troponin I is found in cardiac muscle tissue that weighs 23876 Da. Troponin I is an efficient marker of cardiac muscle tissue injury. Troponin I is a part of a three subunit complex consisting of Troponin T and Troponin C. After cardiac injury happens, Troponin I is released to the blood for 4-6 hours after the pain. cTnI levels stay elevated for 6-10 days. Compared with CK-MB, Troponin I is much more specific in diagnosing myocardial infarction. Troponin I allows determining early if a myocardial injury has occurred or a patient with chest pain has ischemia. It also helps to identify patients 2 days after and 6 days before the infarction, and also identifies false-positive elevations in CK-MB test.
With cTnl Troponin I Test it takes around 10 minutes to see results. The measurement range for the test is between 1 ng/ml to 10 ng/ml.
The test kit is usable until the expiration date if the packaging remains unopened and stored at 2 to 30 °C as it is stated on the label. Do not freeze or use beyond the expiration date.
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- The sensitivity and specificity are fully validated and currently fulfill the basic requirements of rapid diagnostic tests.
cTnI Troponin I test kit can be used with whole blood, serum or plasma.
Collecting whole blood specimens, first, wash the patient’s hands with soap or alcohol and allow the hands to dry. Massage the hands in order to increase blood circulation. Puncture the skin with a sterile lancet and wipe away the first sign of blood. Rub through the hand from wrist to palm finger to form a rounded blood drop on the puncture site.
Then add the whole blood specimen using a capillary tube to the test device. Fill the capillary tube to around 80 µL and avoid air bubbles. Dispense the whole blood into the test kit’s specimen well.
When adding the whole blood specimen to the test device using hanging drops, put the patient’s finger on top of the specimen well and drop 3 drops to fall into the specimen well, or move the finger so that the hanging drops touch the specimen well. Don’t let the finger touch the specimen well. After specimen collection through fingerstick, do the testing immediately.
Serum and plasma specimens can be stored up to 3 days at 2 to 8 °C. For long-term specimen storage use storage temperature below -20°C. However, collecting blood samples by venipuncture should be stored at 2 to 8 °C and tested within 2 days.
Specimens should be brought to room temperature before testing.
- Do not use after expiration date;
- Do not open the sealed pouch until use;
- Do not use if pouch is damaged;
- Humidity and temperature can affect test results.;
- Used test should be discarded according to local regulations.